چکیده

Statement of Problem Plants have great importance in our lives because they fulfill our basic needs. Recently people have used herbs to treat different diseases because they are cheap and effective. Medicinal plants can provide a wealth of antimicrobial agents and hundreds have been investigated for biological activities. Anthocyanins are a group of polyphenolic secondary metabolites also referred to as flavonoids that are frequently found in human diets in the form of plants fruits and vegetables. Anthocyanin has been shown to exhibit a variety of biological functions including anti-oxidant immune-modulatory anti-tumor anti-inflammatory and anti-bacterial effects. Anthocyanins are water-soluble pigments that give several fruits and vegetables including red cabbage beans onions radishes jujube berries grapes and pomegranates their blue purple and red colors. For checked antibacterial effects of anthocyanin in pathogen bacteria we invested Staphylococcus aureus. S.aureus is the bacterium commonly responsible for all major bone and joint infections it can also cause a wide range of illnesses from sepsis and deadly pneumonia to moderately severe skin infections. HA-MRSA or MRSA is a type of methicillin-resistant Staphylococcus aureus infection and is most frequently found in hospitals. This bacterium has another community-acquired strain known as CA-MRSA. Research Purpose Recent studies have investigated the antibiotic effects of anthocyanins so in this study we investigated the antibiotic effect of jujube extract that contains anthocyanin against the pathogen S.aureus. For this reason in this study the alcohol-water method was used to extract jujube extract. Then we evaluated the antibacterial activity of jujube extract using a disc diffusion method in LB agar medium. Research Method We used the homogenize water and ethanol techniques to extract the anthocyanin-containing extract in order to examine the anthocyanin s properties. Escherichia coli and Bacillus subtilis were used in the antibacterial assay of barberry extract products. We use Luria-Bertani Agar medium for this experiment which contains 1.5 g of agar 0.5 g of yeast 1 g of NaCl 1 g of tryptone and 100 ml of distilled water. The diameter of the growth inhibition zone of products containing barberry extract was determined following an 18-hour incubation period in bacterial culture. An uncontaminated area around the bacterium shows that the antibiotic therapy has successfully stopped or inhibited microbial growth. Results and Conclusion The inhibition zones were 19 and 25 mm for two concentrations (7 and 14 μM) of jujube extract against S.aureus respectively implying that jujube extract can inhibit S.aureus growth effectively.

کليدواژه ها

disc diffusion ، Anthocyanins ، Staphylococcus aureus، jujube extract، antibiotic ،clear zone

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ارزو معرفت , 1402 , اثرات ضد باکتریایی عصاره عناب بر علیه استافیلوکوکوس اورئوس , كنگره بين المللي فرآورده‌هاي طبيعي شفابخش