چکیده

Statement of Problem Despite the fact that paclitaxel is a natural effective anticancer agent approved by the FDA and included in the WHO s essential medicines list (EML) it is not widely available due to uneconomical and unsustainable industrial production based on low-growth and low-yielding Taxus trees. The fact is that endophytic paclitaxel-producing fungi will be a cheap and environmentally benign way to scale up industrial production through production enhancements. Research Purpose Increasing paclitaxel production in an endophytic fungus Alternaria alternata isolate MSh Taxus strain isolated from Taxus baccata by employing antifungal agents as fungal envelop destabilizers to leak paclitaxel end products from the cell and reduce feed-back inhibition for paclitaxel production. Research Method Antifungal agents such as clotrimazole and cinnamon methanol extract were added to 5-day-old cultures of Alternaria alternata isolate MSh Taxus strain in submerged fermentation using modified YPD media and incubated for 5 days at standard conditions. The amounts of paclitaxel produced were then determined using an HPLC instrument. Results and Conclusion Cinnamon extract at a concentration of 15 mg ml raised paclitaxel production from 715.46 mg l to 1944.78 mg l whereas clotrimazole at a concentration of 2 g ml boosted paclitaxel production from 759.40 to 1012.09. Therefore treating fungal culture with cinnamon extract will pave the way for microbial industrial production.

کليدواژه ها

Paclitaxel Endophytic fungi Antifungal agent Natural product

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محسن مبینی دهکردی , 1402 , استفاده از عوامل ضد قارچی به عنوان افزاینده پاکلی تاکسل در سویه ی Alternaria alternata isolate MSh Taxus تحت تخمیر غوطه ور , كنگره بين المللي فرآورده‌هاي طبيعي شفابخش